OPTIMIZATION OF GROWTH CONDITIONS AND CHARACTERIZATION OF ENZYMATIC ACTIVITY OF SELECTED NOVEL STREPTOMYCES SPECIES FROM KENYAN SOILS

  • E. N. Karanja Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • H.I boga Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • A.W Muigai Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • F Wamunyokoli Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • J. Kinyua Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • J. O. Nonoh Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
Keywords: Streptomyces, enzymatic index (EI), amylase, lipase, protease, esterase

Abstract

This study was aimed at unveiling and assessing protease, esterase, amylase and lipase enzymes from selected
novel Streptomyces species with biotechnological interest. Four Streptomyces isolates from Jomo Kenyatta
University of Agriculture and Technology farm soil were studied. Physiochemical and biochemical characterization
of the isolates was carried out. The isolates grew well at pH 6, 7, 9 and temperatures of 27.5 oC, 30 oC, and 32.5 oC.
They preferentially required sodium chloride (0 g/l – 17.5 g/l) for growth. All the isolates produced amylase, lipase,
protease and esterase enzymes apart from one isolate that did not produce esterase enzyme.

Published
2019-05-13