GROWTH CHARACTERISTICS AND PRODUCTION OF SECONDARY METABOLITES FROM SELECTED NOVEL STREPTOMYCES SPECIES ISOLATED FROM SELECTED KENYAN NATIONAL PARKS

  • E.N. Karanja, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • H.I Boga Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • A.W. Muigai Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • F. Wamunyokoli Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • J. Kinyua Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • J. O. Nonoh Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya

Abstract

The aim of study was to characterize growth of novel Streptomyces isolates as well as the secondary metabolites
they were producing. Four Streptomyces isolates from Chyulu National Park (Chy 4-10, Chy 15-10, Chy 15-5 and
Chy 2-3) and one from Ruma National Park (Ruj 7-1) were studied. The isolates grew well at pH 6, 7, 9 and
temperatures of 27.5 oC, 30 oC, and 32.5 oC. They preferentially utilized glucose and xylose and also required
sodium chloride (0 g/l – 17.5 g/l) for growth. Antimicrobial products were extracted using ethyl acetate and the
crude secondary metabolite extracts analyzed using Gas Chromatography- Mass Spectrophotometer (GC-MS). 0.54
g/l, 0.62 g/l, 0.41 g/l, 0.3 g/l and 0.14 g/l yields of crude secondary metabolites were extracted from the isolates.
The crude secondary metabolites had different levels of activity against Gram positive and Gram negative test
bacteria. Characterization of the crude secondary metabolites indicated presence of chemical compounds ranging
from amides, amines, acids, pyrrolizidines, butenolides, alcohols and hydrocarbons.

Published
2019-05-13