Facile synthesis of silver and functionalized nanocrystals of Bryophyllum pinnatum and its nano-biotechnological potential

Abstract

Bryophyllum pinnatum is commonly used in trado-medical practices for the treatment of diseases. In this study, silver nanocrystals of B. pinnatum crude methanolic extracts (BpSNCRs) were produced, functionalized with streptomycin (SFBpSNCRs), and its nano biotechnological applications were investigated. The BpSNCRs were characterized using UV-Visible spectroscopic, FTIR, SEM, TEM, TGA, XRD, and EDX techniques, and its dye degradation, bio-flocculating, anti-coagulating potentials, and in–vitro antibacterial efficiency was evaluated using standard methods. Phytosynthesis of spherical shape BpSNCRs was mediated using methanolic extracts of B. pinnatum as bio-reducing and capping agents. The Surface Plasmon Resonance (SPR) peaks were at 400 and 500nm at 24 to 72 hours. The FTIR, SEM, TEM, TGA, XRD, and EDX analyses respectively confirmed silver nanoparticle formation. The functionalized silver nanoparticles had SPR peaks at 600nm. The greenly synthesized BpSNCRs had moderate red Dylon dye degradative efficiency with 49.09% degradation at 0.9 mg/mL after 4 days of exposure. It has 92.3% flocculating efficiency in kaolin suspension and anticoagulant ability of human blood plasma. The BpSNCRs and SFBpSNCRs exhibited in-vitro antibacterial activity against test pathogens, the highest susceptibility was recorded against Vibro cholerae (12 and 28 mm) respectively. B. pinnatum methanolic extracts facilely bio-reduced silver nitrate for silver nanocrystals biosynthesis.